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KMID : 1123920050190041022
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Korean Journal of Oriental Physiology and Pathology
2005 Volume.19 No. 4 p.1022 ~ p.1026
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Effect of Antioxidant and Ampa/kainate Receptor Antagonist on Cerebral Neurons Damaged by Ischemia
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Oh Yeon-Kyun
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Abstract
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To clarify the toxic effect on cultured neonatal mouse cerebral neurons damaged by ischemia, we examined the cytotoxicity induced by ischemia and the protective effect of antioxidant and AMPA/kainate receptor antagonist against ischemia-induced cytotoxicity on cultured cerebral neurons. For this study, mice were administrated with 20ug/kg cyclothiazide or 50U/kg vitamin E via intraperitoneal injection for 2 hours before ischemic induction. After cell culture for 7 days, cell viability, amount of neurofilament and protein kinase C activity were examined. Ischemia decreased significantly cell viability, amount of neurofilament and the increase of protein kinase C activity in these cultures. In the protective effect, vitamin I showed remarkably the increase of cell viability and amount of neurofilament, and the decrease of protein kinase C activity but, cyclothiazide did not showed any protective effect on ischemia-induced cytotoxicity. From these results, it is suggested that vitamin I is effective in blocking the neurotoxicity induced by ischemia, but cyclothiazide as a AMPA/kainate receptor antagonist is not.
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KEYWORD
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Ischemia, Cerebral neuron, Cell viability, Neurofilament, Protein kinase C
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